A Single-Chain Mpox mRNA Vaccine Elicits Protective Immune Response in Mice.

ABSTRACT: Background: The re-emerging mpox virus (MPXV) has spread to numerous countries and raised global concern. There is an urgent need for a safe and effective mRNA vaccine candidate against MPXV infection. Previously, we developed a penta-component mRNA vaccine that contained five distinct antigen-encoded mRNAs encapsulated within lipid nanoparticles (LNPs). Here, we sought to develop a single-chain mRNA vaccine that encodes antigens derived from both intracellular mature virion (IMV) and extracellular enveloped virion (EEV). Methods: A single-chain mRNA vaccine encoding a fusion protein comprising the ectodomains of M1R (eM1R) and A35R (eA35R) (MPXV^eM1-eA35) was developed and characterized, while an admixed formulation of two individual mRNA-LNPs encoding separate ant

SUBMITTER: Xu Q 

PROVIDER: S-EPMC12115521 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature