Mechanisms of Transmission and Adaptation of <i>tet</i>(X4)-Positive IncHI1 Plasmids in XDR <i>Escherichia coli</i> from Pet Dogs: The Role of <i>trhC</i>, <i>rsp</i>, and the Tra1 Region.

ABSTRACT: tet(X4)-positive IncHI1 plasmids are widely prevalent in various bacteria. To understand their transmission characteristics, we analyzed two extensively drug-resistant (XDR) Escherichia coli strains isolated from pet dog feces in Henan Province, China. Strain T28R harbored tet(X4)-positive IncHI1, IncF18:A-:B-, and mcr-1-positive IncI2 plasmids, while T16R carried tet(X4)-positive IncHI1, F16:A-:B-, and mcr-1-positive IncX4 plasmids. Four representative fusion plasmids, pT28R-F1, pT28R-F2, pT28R-F3, and pT16R-F1, in transconjugants were analyzed using WGS and PCR mapping. The results showed that IS26 from the IncF18:A-:B--plasmid attacked the conjugative transfer-associated genes trhc and rsp on the IncHI1 plasmid, generating p

SUBMITTER: Ding P 

PROVIDER: S-EPMC12115866 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature