Reference Whole Genome Sequence Analyses and Characterization of a Novel <i>Carnobacterium maltaromaticum</i> Distinct Sequence Type Isolated from a North American Gray Wolf (<i>Canis lupus</i>) Gastrointestinal Tract.

ABSTRACT: We hypothesize that bacteria isolated from free-ranging animals could potentially be useful for practical applications. To meet this objective a Gram-positive bacterium was isolated from the gastrointestinal (GI) tract of a Gray Wolf (Canis lupus) using Brucella broth with hemin and vitamin K (BBHK). By small ribosomal RNA (16S) gene sequencing the bacterium was initially identified as a novel Carnobacterium maltaromaticum strain. The bacterium could be propagated both anaerobically and aerobically and was both catalase/oxidase negative and negative by the starch hydrolysis as well as negative using lipase assays. The reference whole genome sequence (WGS) was obtained using both Illumina and Nanopore sequencing. The genome assembly was 3,512,202 bp in length, encoding core ba

SUBMITTER: Klews CC 

PROVIDER: S-EPMC12115997 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature