Multi-Omics Unveils Inflammatory Regulation of Fermented Sini Decoction Dregs in Broilers Infected with Avian Pathogenic <i>Escherichia coli</i>.

ABSTRACT: Avian colibacillosis causes significant economic losses and raises concerns for human health due to food safety risks, a problem exacerbated by the increase in antibiotic resistance. This study aimed to develop novel antibacterial strategies using fermented liquid of Sini decoction dregs to address these challenges. We analyzed the transcriptome of the chicken thymus sample GSE69014 in the GEO database to identify immune-related genes, performed molecular docking to assess compound interactions, and experimental validation via Western blot and ELISA to evaluate anti-inflammatory effects. Results revealed 11 core genes, including TLR4, critical for immune responses against the infection, with TLR4 activating key inflammatory pathways. Fermented liquid with probio

SUBMITTER: Mo S 

PROVIDER: S-EPMC12116022 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature