Reduction in cord blood graft potency due to processing delay can be prevented by trehalose supplementation.

ABSTRACT:

Background and objectives

Maximizing the potency of cord blood (CB) units (CBU) is a goal of all CB banks. Low transplant cell dose in CB transplantation is one of several contributors to the slower engraftment. Processing delays could also be at play because CBU can be stored at room temperature (RT) for up to 48 h before cryopreservation. We hypothesized that prolonged storage at RT reduces the potency and engraftment activities of CBU due to the loss of haematopoietic stem cells (HSCs) and progenitor cells.

Materials and methods

CBU were split in half, with one half processed shortly after collection and the other half after 40 h of storage at RT. Viabilities and potencies were compared post thaw, and engraftment activity was tested in xenotransplants. Bioinformatic analy

SUBMITTER: Jahan S 

PROVIDER: S-EPMC12116034 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature