Rapid detection of avipoxvirus using a fluorescent probe-based multienzyme isothermal amplification assay.

ABSTRACT: Avipoxvirus (APV) is a prevalent DNA virus in avian species, causing clinical symptoms of fowlpox and leading to reduced egg production, slower broiler growth, and increased mortality. The spread of APV poses a significant threat to the global poultry industry, potentially causing substantial economic losses. Effective control of APV, particularly its major species such as fowlpoxvirus and pigeonpoxvirus, requires the development of rapid and specific diagnostic tools. In this study, a novel multi-enzyme isothermal rapid amplification (MIRA) assay was developed to detect APV. Various primer-probe combinations were screened to identify an optimal pair targeting a conserved region of the viral P4b gene. The MIRA assay operates at a constant temperature and results can be visualized th

SUBMITTER: Zhu Y 

PROVIDER: S-EPMC12116516 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature