Intercellular communication between FAP+ fibroblasts and SPP1+ macrophages in prostate cancer via multi-omics.

ABSTRACT:

Background

Prostate cancer (PCa) presents substantial heterogeneity and unpredictability in its progression. Despite therapeutic advancements, mortality from advanced PCa remains a significant challenge. Understanding the intercellular communication within the tumor microenvironment (TME) is critical for uncovering mechanisms driving tumorigenesis and identifying novel therapeutic targets.

Methods

We employed an integrative approach combining bulk RNA sequencing, single-cell RNA sequencing (scRNA-seq), and spatial transcriptomics to investigate interactions between FAP+ fibroblasts and tumor-associated macrophages in PCa. Key findings were validated using immunohistochemical and immunofluorescence staining techniques.

Results

Analysis of 23,519 scRNA-seq data from 23

SUBMITTER: Wu T 

PROVIDER: S-EPMC12116517 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature