Whole-genome probe capture sequencing reveals genomic diversity and characteristics of <i>Mycoplasma pneumoniae</i> in Nanjing, China.

ABSTRACT: Mycoplasma pneumoniae (M. pneumoniae), a slow-growing, fastidious Gram-negative bacterium and a leading cause of community-acquired pneumonia globally, remains understudied and underreported across numerous geographical areas in China despite its worldwide significance. This study employed probe capture sequencing for targeted enrichment and direct sequencing of M. pneumoniae from clinical samples, combined with comparative genomic analyses of contemporary and historical global genomes. Core genome and pan-genome revealed that the M. pneumoniae genomes were classified into two distinct clades, P1-I and P1-II, each associated with a specific sequence type (ST). Most of the genomes sequenced in this study were identified as P1-I (86.96%, 20/23), contrasting with t

SUBMITTER: Zhou G 

PROVIDER: S-EPMC12116587 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature