Profiling Serine Hydrolases in the Leishmania Host-Pathogen Interactome Using Cell-Permeable Activity-Based Fluorophosphonate Probes.

ABSTRACT: Leishmaniasis, a vector-borne neglected tropical disease, caused by the protozoan parasite Leishmania, is a major global public health challenge with millions of new cases annually. Treatment of leishmaniasis is difficult for many reasons including multiple lifecycle stages, involving both an infective insect vector form, the promastigote, and a disease-causing intracellular mammalian host form, the amastigote, and increasing drug tolerance that are all linked by the interplay between parasite and host. Activity-based protein profiling (ABPP) was employed using new cell-permeable fluorophosphonate probes to explore serine hydrolases (SHs) in Leishmania mexicana with subsequent analysis enabled by secondary reaction with an affinity reagent. Importantly, these cell-permeable probes are capa

SUBMITTER: Isern JA 

PROVIDER: S-EPMC12117450 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature