In-Depth Chemical Analysis of the Brain Extracellular Space Using <i>In Vivo</i> Microdialysis with Liquid Chromatography-Tandem Mass Spectrometry.

ABSTRACT: Metabolomic analysis of samples acquired in vivo from the brain extracellular space by microdialysis sampling can provide insights into chemical underpinnings of a given brain state and how it changes over time. Small sample volumes and low physiological concentrations have limited the identification of compounds from this compartment, so at present, we have scant knowledge of its composition. As a result, most in vivo measurements have limited depth of analysis. Here, we describe an approach to (1) identify hundreds of compounds in brain dialysate and (2) routinely detect many of these compounds in 5 μL microdialysis samples to enable deep monitoring of brain chemistry in time-resolved studies. Dialysate samples collected over 12 h were concentrated 10-fold and then analyzed

SUBMITTER: Anderson BG 

PROVIDER: S-EPMC12117515 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature