Semaglultide targets Spp1⁺ microglia/macrophage to attenuate neuroinflammation following perioperative stroke.

ABSTRACT: Peripheral surgery evokes neuroimmune activation in the central nervous system and modulates immune cell polarization in the ischemic brain. However, the phenotypic change of microglia and myeloid cells within post-surgical ischemic brain tissue remain poorly defined. Using an integrated approach that combines single-cell RNA sequencing with comprehensive biological analysis in a perioperative ischemic stroke (PIS) model, we identified a distinct Spp1-positive macrophage/microglia (Spp1⁺ Mac/MG) subgroup that exhibit enriched anti-inflammatory pathways with distinct lipid metabolic reprogrammed profile. Moreover, using immunofluorescence staining, we identified the expression of Glucagon-like peptide-1 receptor (GLP1R) in Spp1⁺F4/80⁺ cells and Spp1⁺

SUBMITTER: Li Y 

PROVIDER: S-EPMC12117698 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature