Sheep immune response against a novel recombinant enterotoxemia and infectious necrotic hepatitis vaccine in Turkiye.

ABSTRACT:

Background

Clostridial diseases are common in ruminants all over the world. This study investigated the efficacy of a novel recombinant vaccine developed against enterotoxemia and infectious necrotic hepatitis in sheep.

Results

Plasmids carrying the Clostridium novyi type B alpha toxin gene (CnBtcn-alpha), Clostridium perfringens type C beta toxin gene (CpCcpb) and C. perfringens type D epsilon toxin gene (CpDetx) were constructed and the plasmids were transferred to Escherichia coli. Unpurified protein obtained from E. coli cell lysate supernatant was used to prepare the recombinant vaccine. The vaccine was prepared in three different formulas (RV100, RV200 and RV400), with 100, 200 and 400 µg of each protein in one vaccine dose. RV400 was preferred to immunise sheep as the

SUBMITTER: Ekinoglu DN 

PROVIDER: S-EPMC12117762 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature