Integrated in-silico design and in vivo validation of multi-epitope vaccines for norovirus.

ABSTRACT:

Background

Norovirus (NoVs) is a foodborne pathogen that causes acute gastroenteritis. The diversity of its principal antigenic protein poses a significant challenge to vaccine development and the prevention of large-scale outbreaks globally. Currently, no licensed vaccines against norovirus have been approved.

Methods

We developed a novel pipeline that integrates multiple bioinformatics tools to design broad-spectrum vaccines against NoVs. Specifically, broad-spectrum T-cell epitope vaccines were designed based on consensus sequences and optimized epitope screening, while broad-spectrum B-cell spatial epitope vaccines were constructed using high-throughput antigenicity calculations and epitope mapping.

Results

This pipeline underwent rigorous validation at three leve

SUBMITTER: Qiu J 

PROVIDER: S-EPMC12117790 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature