Discovery and In Vitro Reconstitution of Closoxazole Biosynthesis from Pyxidicoccus fallax.

ABSTRACT: Benzoxazoles are important structural components of both bioactive natural products and pharmaceutical active ingredients. In this study, a putative benzoxazole gene cluster originating from the myxobacterium Pyxidicoccus fallax is investigated. This gene cluster is found to confer the ability for production of closoxazoles, which were recently discovered in the anaerobic bacterium Clostridium cavendishii. To obtain further insights into the biosynthetic mechanism, the required key enzymes are subjected to in vitro studies. Notably, significant differences to the biosynthetic pathway in C. cavendishii are observed. First, the condensing amidohydrolase uses an unstable ester as substrate and, thus, establishes a CN bond for benzoxazole formation. In contrast, the homolog from C. cavendishi

SUBMITTER: Lernoud L 

PROVIDER: S-EPMC12118335 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature