Single-Cell RNA Sequencing of the Primary Visual Cortex in Mice With Optic Nerve Injury.

ABSTRACT:

Purpose

Glial cells play a critical role in primary visual cortex (V1 region) damage caused by optic nerve injury, but the mechanisms driving progression of V1 region injury and glial cell heterogeneity remain poorly understood. This study aimed to investigate the damage changes in the V1 region of mice after optic nerve crush (ONC) by single-cell RNA sequencing (scRNA-seq).

Methods

Hematoxylin and eosin (H&E) and immunofluorescence staining were used to evaluate the changes of retinal thickness, astrocytes, and microglia in the V1 region after ONC in mice. Single cell suspensions in the V1 region of mice were prepared and analyzed by scRNA-seq with Seurat, cellchat, CytoTRACE in R software. The expression of PTGDS and CRYAB was measured by qPCR, Western blot, and immunofluo

SUBMITTER: Li D 

PROVIDER: S-EPMC12118511 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature