Coding principles of dopaminergic transmission modes.

ABSTRACT: Dopaminergic neurons influence diverse behaviors with varied firing patterns, yet the precise mechanisms remain unclear. We introduce a multiplexed genetically encoded sensor-based imaging and voltammetry method to simultaneously record synaptic, perisynaptic, and extrasynaptic dopaminergic transmission at mouse central neurons. Using this method alongside a genetically encoded sensor-based image analysis program, we found that heterogeneous dopaminergic firing patterns create various transmission modes, encoding frequency, number, and synchrony of firing pulses using neurotransmitter quantity, releasing synapse count, and synaptic and/or volume transmission. Under both tonic and low-frequency phasic activities, transporters effectively reuptake dopamine at perisynaptic sites, confining do

SUBMITTER: Huang L 

PROVIDER: S-EPMC12118558 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature