Proliferative arrest induces neuronal differentiation and innate immune responses in normal and Creutzfeldt-Jakob Disease agent (CJ) infected rat septal neurons.

ABSTRACT: Rat post-mitotic septal neurons, engineered to reversibly proliferate and arrest under physiological conditions, can be maintained for weeks without cytotoxic effects. Nine representative independent cDNA libraries were made to evaluate global arrest-induced neural differentiation and innate immune responses, e.g., upregulated interferon (β-IFN) RNA, that were previously identified in normal uninfected and Creutzfeldt-Jakob Disease agent (CJ) infected septal neurons. This reversible cell model encompassed a non-productive latent (CJ-) and a highly infectious (CJ + , 10 logs/gm) state. Arrest of normal uninfected neurons upregulated a plethora of anti-proliferative transcripts and known neuronal differentiation transcripts (e.g., Neuregulin-1, GDF6 and Prnp). As expected, many activated IFN

SUBMITTER: Pagano N 

PROVIDER: S-EPMC12118874 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature