Mycobacterium tuberculosis curli pili (MTP) and heparin-binding hemagglutinin adhesin (HBHA) facilitate regulation of central carbon metabolism, enhancement of ATP synthesis and cell wall biosynthesis.

ABSTRACT: Functional 'omics' studies previously identified the M. tuberculosis surface located adhesins, heparin-binding hemagglutinin adhesin (HBHA) and curli pili (MTP) as significant potential targets for the design of tuberculosis (TB) point-of-care diagnostics, effective drugs, and vaccines. Little is known on the effect of these adhesins on the pathogen's transcriptome. The current study, via transcriptomics, elucidated whether the deletion of the single genes, hbhA and mtp, and double genes, hbhA-mtp, via specialised transduction, affected global bacterial gene expression. RNA sequencing of M. tuberculosis wild-type V9124 (WT), single and double deletion HBHA and MTP mutant strains were confirmed by reverse transcription quantitative polymerase chain reaction (RT-qPCR) on selected genes, and

SUBMITTER: Naidoo TJ 

PROVIDER: S-EPMC12119724 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature