Three-dimensional genome architecture in intrahepatic cholangiocarcinoma.

ABSTRACT:

Purpose

Intrahepatic cholangiocarcinoma (ICC) is a common primary hepatic tumors with a 5-year survival rate of less than 20%. Therefore, it is crucial to elucidate the molecular mechanisms of ICC. Recently, the advance of high-throughput chromosome conformation capture (Hi-C) technology help us look insight into the three-dimensional (3D) genome structure variation during tumorigenesis. However, its function in ICC pathogenesis remained unclear.

Methods

Hi-C and RNA-sequencing were applied to analyze 3D genome structures and gene expression in ICC and adjacent noncancerous hepatic tissue (ANHT). Furthermore, the dysregulated genes due to 3D genome changes were validated via quantitative real-time PCR and immunohistochemistry.

Results

Primarily, the intrachromosomal i

SUBMITTER: Liang Y 

PROVIDER: S-EPMC12119775 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature