Use of PCR cycle threshold values for toxins A and B quantification in Clostridioides difficile infections.

ABSTRACT: Increasingly, tcdA/tcdB qPCR is being used to diagnose Clostridioides difficile infections (CDI). Under the hypothesis that toxin genes' quantification through Ct values could potentially improve clinical accuracy, this study aimed to assess the linearity of the C. difficile assay on the QIAstat-Dx^® Gastrointestinal Panel 2 (GI2 Panel) and to correlate bacterial to toxins load. Four analytical and thirty-five clinical toxigenic C. difficile isolates were quantified using three validated standard curve qPCR assays targeting adK, tcdA and tcdB genes. Of these, twelve were then tested to characterize the linearity of the C. difficile assay on the QIAstat-Dx^® GI2 Panel. Statistical analysis of the Ct values of adK, tcdA and tcdB obtained from standard curves presented an

SUBMITTER: Smith-Aguasca R 

PROVIDER: S-EPMC12119821 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature