Functional Relationships of Two NFU Proteins in Maintaining the Abundances of Mitochondrial Iron-Sulfur Proteins.

ABSTRACT: Iron-sulfur clusters are involved in many biological processes, including photosynthetic electron transport in the chloroplast and respiratory electron transport in the mitochondrion. Iron-sulfur cluster biosynthesis requires iron-sulfur carriers such as nitrogen-fixation-subunit-U [NFU]-type proteins. The Arabidopsis thaliana nuclear genome encodes two mitochondrion-targeted NFU proteins: NFU4 and NFU5, previously reported to have a primary role in the biosynthesis of the lipoate cofactor, mediated by the 4Fe-4S enzyme lipoyl synthase. Through in vitro reconstitution and spectroscopic analysis, we found that recombinant NFU4 and NFU5 proteins had UV-visible features characteristic of 4Fe-4S clusters. In addition, we confirmed that double homozygous, complete loss-of-function nfu

SUBMITTER: Zhao J 

PROVIDER: S-EPMC12120262 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature