From Invaginating Site to Deep Lesion: Spatial Transcriptomics Unravels Ectopic Endometrial Penetration Features in Adenomyosis.

ABSTRACT: Adenomyosis, characterized by clinical intractability, significantly impacts female fertility and life quality due to the absence of definitive diagnostic markers and effective treatment options. The invagination theory is a primary hypothesis for adenomyosis, but the underlying molecular mechanisms remain unclear. In this study, a spatial transcriptional landscape of adenomyosis with an evident invagination structure is mapped from the endometrial invaginating site to ectopic lesions utilizing spatial transcriptomics and single-cell RNA sequencing. In addition, the authors employ bulk RNA sequencing deconvolution to assess the significance of core spatial ecotypes, use histological techniques to target specific cell types, and conduct in vitro experiments for validation. At the invaginati

SUBMITTER: Li B 

PROVIDER: S-EPMC12120721 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature