Genetic engineering of E. coli K-12 for heterologous carbohydrate antigen production.

ABSTRACT:

Background

Carbohydrate-based vaccines have made a remarkable impact on public health over the past three decades. Efficient production of carbohydrate antigens is a crucial prerequisite for the development of such vaccines. The enzymes involved in the synthesis of bacterial surface carbohydrate antigens are usually encoded by large, uninterrupted gene clusters. Non-pathogenic E. coli glycoengineering starts with the genetic manipulation of these clusters. Heterologous gene cluster recombination through an expression plasmid has several drawbacks, including continuous antibiotic selection pressure, genetic instability, and metabolic burdens. In contrast, chromosome-level gene cluster expression can minimize the metabolic effects on the host and reduce industrial costs.

Results</

SUBMITTER: Li C 

PROVIDER: S-EPMC12121013 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature