A robust in-vitro and ex-vitro Agrobacterium rhizogenes-mediated hairy root transformation system in mungbean for efficient visual screening of transformants using the RUBY reporter.

ABSTRACT:

Background

Mungbean is one of the most economically important grain legume crops in Asia. Functional genomics studies in mungbean are necessary to understand the molecular mechanisms behind agronomic traits, to advance the crop improvement. However, this progress is significantly impeded by the absence of effective and extensive genetic analysis tools. Agrobacterium rhizogenes-mediated hairy root transformation has become a powerful tool for studying gene function and an efficient alternative for investigating root-specific interactions and processes in different species, due to its quick and simple methodology. Agrobacterium-mediated plant transformation, however, is known to be difficult in legumes, especially in mungbean.

Results

In this report, we developed an Agrobacter

SUBMITTER: Kumar S 

PROVIDER: S-EPMC12121287 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature