Multi-Omic Analysis Reveals Astrocytic Annexin-A2 as Critical for Network-Level Circadian Timekeeping in the Suprachiasmatic Nucleus.

ABSTRACT: The mammalian suprachiasmatic nucleus (SCN) orchestrates daily (circadian) rhythms of physiology and behavior by broadcasting timing cues generated autonomously by its mutually reinforcing network of ~10,000 neurons and ~3000 astrocytes. Although astrocytic control of extracellular glutamate and GABA has been implicated in driving circadian oscillations in SCN gene expression and neuronal activity, the full scale of the network-level signaling mechanisms is unknown. To understand better how this astrocyte-neuron network operates, we adopted a multi-omics approach, first using SILAC-based mass spectrometry to generate an SCN proteome where ~7% of identified proteins were circadian. This circadian proteome was analyzed bioinformatically alongside existing single-cell RNAseq transcriptomic da

SUBMITTER: Patton AP 

PROVIDER: S-EPMC12121465 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature