Identification of Soy-Derived Peptides With Micelle Disruption Activity of Secondary Bile Acids.

ABSTRACT: Bile acids, which originate in the liver as primary bile acids, facilitate the absorption of lipophilic components into the intestines and exist as micellar structures. Intestinal bacteria convert primary bile acids to secondary bile acids, such as deoxycholic acid (DCA), which is associated with colorectal cancer. Therefore, their elimination from the body is preferred. Soy protein hydrolysates are known for their bile acid-binding properties, which prompted our investigation of DCA-specific binding and micelle-disrupting peptides. We designed a peptide library from eight soy protein sequences and selected 42 candidate peptides based on their DCA-binding activity. These peptides were synthesized, and their ability to disrupt bile acid micelles was assessed using a 96-well high-throughput

SUBMITTER: Shimizu S 

PROVIDER: S-EPMC12121512 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature