Identification and validation of intergeneric hybrids between Saccharum officinarum and Erianthus rockii using molecular and cytogenetic tools.

ABSTRACT: Sugarcane (Saccharum spp.), a vital crop for sugar and bioenergy production, faces challenges in breeding due to its narrow genetic base and susceptibility to environmental stresses. To enhance genetic diversity, distant hybridization with wild relatives such as Erianthus rockii, known for its drought tolerance and strong ratooning ability, offers a promising strategy. However, identifying true intergeneric hybrids remains a critical challenge. This study identified true intergeneric hybrids from crosses between S. officinarum and E. rockii using tetra-primer ARMS-PCR and HRM curve analysis targeting SNPs in the nrDNA-ITS region, with genomic in situ hybridization (GISH) validating chromosome composition. The results confirmed 13 true hybrids among 16 progeny, showing a chromosome inherita

SUBMITTER: Wang G 

PROVIDER: S-EPMC12121808 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature