Disulfide bond-related gene signature development for bladder cancer prognosis prediction and immune microenvironment characterization.

ABSTRACT: Bladder cancer is the fourth most common malignant tumor in men, with limited therapeutic biomarkers and heterogeneous responses to immunotherapy. Disulfide bond-driven cell death has emerged as a critical regulator of tumor progression and immune microenvironment remodeling. By integrating data from TCGA and GEO cohorts, we developed a Disulfide-Related Prognostic Signature (DRPS) using ten machine learning algorithms. Single-cell RNA sequencing (scRNA-seq) elucidated the cell subtype-specific expression patterns of disulfide bond regulatory genes, while immune microenvironment and drug sensitivity analyses validated its clinical translational potential. qRT-PCR experiments confirmed differential expression patterns of core genes in bladder cancer cell lines. The DRPS model, optimized by

SUBMITTER: Huang H 

PROVIDER: S-EPMC12122888 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature