Cloning and expression of chromate reductase from Bacillus paramycoides S48 for chromium remediation.

ABSTRACT: This study explores the production and characterization of chromium reductase from Bacillus paramycoides S48, focusing on its ability to effectively reduce toxic hexavalent chromium to less harmful chromium. The strain exhibited 65% reduction in Cr(VI) within 96 h at 30 °C. Clear morphological and functional group shifts on strain S48 cell surface treated with metal were noted using analytical tools i.e. SEM-EDX and FT-IR. The physico-chemical conditions such as temperature, pH and nutritional factors were optimized for better chromate reductase yield using Placket-Burman and Central Composite design software. The purified chromate reductase, obtained through size exclusion column chromatography, demonstrated a specific activity of 1416.5 U/mg, and 6.6-fold increase in purity, with a molec

SUBMITTER: Kalsoom K 

PROVIDER: S-EPMC12123004 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature