A Pentatricopeptide Repeat Protein Restores Fertility in Tadukan-Type Cytoplasmic Male Sterile Rice via the Cleavage of the Mitochondrial orf312 RNA.

ABSTRACT: Cytoplasmic male sterility (CMS) is associated with the inhibition of pollen and/or anther development regulated by a CMS-causing gene in the mitochondrial genome; it is a useful trait for preventing self-pollination and producing F₁ hybrids, which can boost crop yields. Pollen and/or anther development can be recovered by the action of the RESTORER OF FERTILITY (Rf) gene, a nuclear-encoded gene. Most reported Rf genes encode pentatricopeptide repeat (PPR) proteins, which bind to RNA and promote RNA processing of the respective CMS-causing gene. In this study, we report the map-based cloning of the Rf gene (Rfta) for Tadukan-type CMS (TA-CMS) in rice (Oryza sativa L.), with anther dehiscence and seed setting inhibited by the mitochondrial gene orf312. The Rfta locus was delimite

SUBMITTER: Takatsuka A 

PROVIDER: S-EPMC12123059 | biostudies-literature | 2025 May-Jun

REPOSITORIES: biostudies-literature