DNMT3A-dependent DNA methylation shapes the endothelial enhancer landscape.

ABSTRACT: DNA methylation plays a fundamental role in regulating transcription during development and differentiation. However, its functional role in the regulation of endothelial cell (EC) transcription during state transition, meaning the switch from an angiogenic to a quiescent cell state, has not been systematically studied. Here, we report the longitudinal changes of the DNA methylome over the lifetime of the murine pulmonary vasculature. We identified prominent alterations in hyper- and hypomethylation during the transition from angiogenic to quiescent ECs. Once a quiescent state was established, DNA methylation marks remained stable throughout EC aging. These longitudinal differentially methylated regions correlated with endothelial gene expression and highlighted the recruitment of de novo

SUBMITTER: Gehrs S 

PROVIDER: S-EPMC12123414 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature