Comprehensive nucleoside analysis of archaeal RNA modification profiles reveals an m⁷G in the conserved P loop of 23S rRNA.

ABSTRACT: Extremophilic Archaea employ diverse RNA modifications for survival. Our understanding of the modified nucleosides and their functions in Archaea is far from complete. Here, we establish an extensive profile of nucleoside modifications in thermophilic and mesophilic Archaea. Through liquid chromatography-tandem mass spectrometry (LC-MS/MS) and rigorous non-coding RNA depletion, we identify four previously unannotated modifications in archaeal mRNA. Nucleoside analysis conducted on total, large, small, and mRNA-enriched subfractions of hyperthermophile Thermococcus kodakarensis reveals modifications whose relative abundance is dynamically responsive to growth temperatures. To predict archaeal RNA-modifying enzymes, we leverage open-access databases to compare putative functional domains wit

SUBMITTER: Tsai YL 

PROVIDER: S-EPMC12124282 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature