NAD⁺/Nrf2 signaling promotes osteogenesis by regulating oxidative level of BMSCs under mechanical stress.

ABSTRACT:

Background

Mechanical stress triggers an increase in cellular reactive oxygen species (ROS), which is associated with the impairment of osteogenesis. During orthodontic treatment, bone marrow mesenchymal stem cells (BMSCs) experience mechanical stress, yet the oxidative profile and redox regulatory mechanisms under such stress, especially involving Nicotinamide adenine dinucleotide (NAD⁺), are not well understood, necessitating further research into their roles in orthodontic therapies.

Methods

The Tension System was established to detect ROS changes in BMSCs under cyclic stretch stress, with H₂O₂ simulating uncontrolled ROS. Flow cytometry and fluorescence staining measured ROS, while an NAD⁺/NADH assay kit assessed NAD⁺

SUBMITTER: Ren H 

PROVIDER: S-EPMC12125440 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature