Transcriptomic profiling after B cell depletion reveals central and peripheral immune cell changes in multiple sclerosis.

ABSTRACT: Multiple sclerosis (MS) is a complex, genetically mediated autoimmune disease of the CNS, in which anti-CD20-mediated B cell depletion is remarkably effective in the treatment of early disease. Although previous studies investigated the effect of B cell depletion on select immune cell subsets using flow cytometry-based methods, the therapeutic effect on the patient's immune landscape is unknown. In this study, we explored how B cell-depleting therapies modulate the immune landscape using single-cell RNA-Seq. We demonstrate that B cell depletion led to cell-type-specific changes in the abundance and function of cerebrospinal fluid (CSF) macrophages and peripheral blood monocytes. Specifically, a CSF-specific macrophage population with an antiinflammatory transcriptomic signature and periphe

SUBMITTER: Wei J 

PROVIDER: S-EPMC12126227 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature