Development of a semicomprehensive detection method for paramyxoviruses and its validation using Indonesian bats.

ABSTRACT: An outbreak of zoonotic diseases is one of the worldwide threats. Bats were reported as important reservoir hosts for many emerging zoonotic diseases. To mitigate the risk, understanding bat virome and their distribution is indispensable. Universal detection methods that can simultaneously identify multiple viruses are some of the most promising approaches. Here, we developed a semicomprehensive detection method integrating group-wide RT-PCR for paramyxoviruses and multiplex next-generation sequencing. The RT-PCR consists of three sets of degenerative primers covering viruses from Paramyxoviridae, including Pneumoviridae, which have now been reclassified into a distinct family. Index nucleotides were added to the primers to enable cost-effective multiplex sequencing, and the length of inde

SUBMITTER: Nesti DR 

PROVIDER: S-EPMC12126574 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature