Charting unknown metabolic reactions by mass spectrometry-resolved stable-isotope tracing metabolomics.

ABSTRACT: Metabolic reactions play important roles in organisms such as providing energy, transmitting signals, and synthesizing biomacromolecules. Charting unknown metabolic reactions in cells is hindered by limited technologies, restricting the holistic understanding of cellular metabolism. Using mass spectrometry-resolved stable-isotope tracing metabolomics, we develop an isotopologue similarity networking strategy, namely IsoNet, to effectively deduce previously unknown metabolic reactions. The strategy uncovers ~300 previously unknown metabolic reactions in living cells and mice. Specifically, we elaborately chart the metabolic reaction network related to glutathione, unveiling three previously unreported reactions nestled within glutathione metabolism. Among these, a transsulfuration reaction,

SUBMITTER: Gao Y 

PROVIDER: S-EPMC12126588 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature