Estrogen receptor β activation alleviates inflammatory bowel disease by suppressing NLRP3-dependent IL-1β production in macrophages via downregulation of intracellular calcium level.

ABSTRACT:

Introduction

Although several estrogen receptor β (ERβ) agonists have been reported to alleviate IBD, the pivotal mechanism remains obscure.

Objectives

To examine the effects and mechanisms of ERβ activation on cytokine/chemokine networks in colitis mice.

Methods

Dextran sulfate sodium salt (DSS) and trinitro-benzene-sulfonic acid (TNBS) were used to induce mouse colitis model. Multiple molecular biological methods were employed to evaluate the severity of mouse colitis and the level of cytokine and/or chemokine.

Results

Bioinformatics analysis, ELISA and immunofluorescence results showed that the targeted cytokines and/or chemokines associated with ERβ expression and activation is IL-1β, and the anti-colitis effect of ERβ activation was significantly attenuate

SUBMITTER: Zhu Y 

PROVIDER: S-EPMC12126700 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature