GYY4137-induced p65 sulfhydration protects synovial macrophages against pyroptosis by improving mitochondrial function in osteoarthritis development.

ABSTRACT:

Introduction

Osteoarthritis (OA) is the most common arthritis that is characterized by the progressive synovial inflammation and loss of articular cartilage. Although GYY4137 is a novel and slow-releasing hydrogen sulfide (H₂S) donor with potent anti-inflammatory properties that may modulate the progression of OA, its underlying mechanism remains unclear.

Objectives

In this study, we validated the protective role of GYY4137 against OA pathological courses and elucidated its underlying regulatory mechanisms.

Methods

Cell transfection, immunofluorescence staining, EdU assay, transmission electron microscopy, mitochondrial membrane potential measurement, electrophoretic mobility shift assay, sulfhydration assay, qPCR and western blot assays were performed in t

SUBMITTER: Ma J 

PROVIDER: S-EPMC12126715 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature