WTAP/YTHDF1-mediated m⁶A modification amplifies IFN-γ-induced immunosuppressive properties of human MSCs.

ABSTRACT:

Introduction

The immunosuppressive capacity of mesenchymal stem cells (MSCs) is dependent on the "license" of several pro-inflammatory factors to express immunosuppressive molecular profiles, which determines the therapeutic efficacy of MSCs in immune-mediated inflammatory diseases. Of those, interferon-γ (IFN-γ) is a key inducer for the expression of immunosuppressive molecular profiles; however, the mechanism underlying this effect is unknown.

Objectives

To elucidate the regulation mechanism and biological functions of N⁶-methyladenosine (m⁶A) modification in the immunosuppressive functions by the IFN-γ-licensing MSCs.

Methods

Epitranscriptomic microarray analysis and MeRIP-qPCR assay were performed to identify the regulatory effect of WTAP in

SUBMITTER: Chen Q 

PROVIDER: S-EPMC12126738 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature