Alkane biosynthesis gene expression and its increased production in recombinant cyanobacteria.

ABSTRACT: Microalgae such as cyanobacteria convert CO₂ to compatible drop-in fuels, such as alkanes. However, the production yield is approximately 0.05-1.0% of the dry weight of natural algae. Here, we aimed to study the role of transcriptional expression, mRNA molecular structure and culture-dependent accumulation of alkanes from two cyanobacteria species. The transcription start sites of the alkane biosynthesis genes ado and aar were identified in the representative cyanobacteria strains Synechocystis sp. PCC 6803 and Limnothrix sp. SK1-2-1, which produce heptadecane and pentadecane, respectively. This characterisation revealed the potential promoters and unique mRNA structures of the ado and aar genes in these species. Transcripts from these genes were induced more in the nitrogen-dep

SUBMITTER: Nagao M 

PROVIDER: S-EPMC12127877 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature