Human microbiota influence the immune cell composition and gene expression in the tumor environment of a murine model of glioma.

ABSTRACT:

Background

Immunotherapy has shown success against other cancers but not glioblastoma. Previous data has revealed that microbiota influences anti-PD-1 efficacy. We have previously found that, when using gnotobiotic mice transplanted with human fecal microbiota, the gut microbial composition influenced the response to anti-PD-1 in a mouse model of glioma. However, the role of the human microbiota in influencing the mouse immune cells in the glioma microenvironment and anti-PD-1 response was largely unknown. Using two distinct humanized microbiome (HuM) lines, we used single-cell RNA sequencing (scRNA-seq) to determine how gut microbiota affect immune infiltration and gene expression in a murine glioma model.

Methods

16S rRNA sequencing was performed on fecal samples from HuM1

SUBMITTER: Green GBH 

PROVIDER: S-EPMC12128662 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature