Environmentally derived Balamuthia mandrillaris contains endosymbiotic bacteria.

ABSTRACT: Balamuthia mandrillaris is an environmentally derived, free-living amoeba that causes fatal meningoencephalitis. We previously isolated B. mandrillaris from soil in the Aomori Prefecture and attempted to culture the cell-free amoeba using liquid medium; however, this was difficult to achieve because of contamination of the medium with endogenous bacteria. The aim of this study was to determine the presence of endogenous bacteria in environmentally derived B. mandrillaris and identify bacteria. Two new environmentally derived B. mandrillaris strains were isolated from soil samples collected throughout Japan. Environmentally derived B. mandrillaris was cultured under nutrient-free conditions for 60 days, and the induced cysts contained large amounts of viable bacteria. The sequence of the en

SUBMITTER: Yamanouchi K 

PROVIDER: S-EPMC12130064 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature