Multiparametric functional characterization of individual lipid nanoparticles using surface-sensitive light-scattering microscopy.

ABSTRACT: The most efficient lipid nanoparticles (LNPs) for gene therapeutics rely on specific lipids that protect the oligonucleotide cargo and aid cellular uptake and subsequent endosomal escape. Yet, the efficacy of current state-of-the-art LNP formulations remains low, a few percent at best. A deeper understanding of how LNP cargo, lipid composition, stoichiometry, size, structure, and pH-induced conformational changes influence their efficiency is therefore necessary for improved design. Given the variability of these properties, preferred screening methods should offer single-particle-resolved multiparametric characterization. In this work, we employ combined surface-sensitive fluorescence and label-free scattering microscopy with single LNP resolution, which when integrated with microfluidics

SUBMITTER: Sjoberg M 

PROVIDER: S-EPMC12130878 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature