The contribution of the Golgi and the endoplasmic reticulum to calcium and pH homeostasis in Toxoplasma gondii.

ABSTRACT: The cytosolic Ca²⁺ concentration of all cells is highly regulated demanding the coordinated operation of Ca²⁺ pumps, channels, exchangers, and binding proteins. In the protozoan parasite Toxoplasma gondii, calcium homeostasis, essential for signaling, governs critical virulence traits. However, the identity of most molecular players involved in signaling and homeostasis in T. gondii is unknown or poorly characterized. In this work, we studied a putative calcium proton exchanger, TgGT1_319550 (TgCAXL1), which belongs to a family of Ca²⁺/proton exchangers that localize to the Golgi apparatus. We localized TgCAXL1 to the Golgi and the endoplasmic reticulum (ER) of T. gondii and validated its role as a Ca²⁺/proton exchanger by yeast complementation.

SUBMITTER: Calixto A 

PROVIDER: S-EPMC12131190 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature