Circ_0002456/FUS interaction inhibits NF-κB signaling to attenuate DNA damage and inflammatory responses in hDPSCs.

ABSTRACT:

Objectives

circular RNAs (circRNAs) are emerging regulators of inflammatory diseases, but their role in pulpitis remains unclear. This study aimed to investigate the role of circRNA in the occurrence and development of pulpitis.

Methods

qRT-PCR and Western Blot were used to detect γ-H2AX, a marker of DNA double-strand breaks (DSBs), and inflammatory factors in pulp tissues. Bioinformatics identified dysregulated circRNAs. Loss- and gain-of-function experiments were conducted to explore the function of circ_0002456 in lipopolysaccharide (LPS)-induced DNA damage and inflammation in human dental pulp stem cells (hDPSCs). The interaction between circ_0002456 and fused in sarcoma (FUS) protein was validated by RNA fluorescence in situ hybridization (FISH), RNA pull-down and nucle

SUBMITTER: Lin L 

PROVIDER: S-EPMC12131344 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature