Genetically engineered bacteria and microalgae expressing a mutant of cytochrome P450 BM3 for efficient Diuron degradation in wastewater treatment.

ABSTRACT: The diverse members of the cytochrome P450 superfamily conduct the metabolism of a wide variety of compounds, both endogenous and xenobiotic, thus offering potential for degrading emerging contaminants such as prescription drugs and pesticides in wastewater treatment and aquaculture. This study demonstrates the expression and activity of the mutant P450 enzyme of CYP102A1 (BM3 MT35) engineered in Bacillus megaterium and Chlamydomonas reinhardtii, harnessed for the degradation of the herbicide Diuron. The P450 BM3 MT35 enzyme was transgenically expressed in Escherichia coli and purified via a His6-tag system, with its activity confirmed by spectral and in vitro metabolism assays. During in vivo experiments, transgenic Bacillus megaterium degraded 23

SUBMITTER: Helvig C 

PROVIDER: S-EPMC12131741 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature