The soil microorganism <i>Bacillus amyloliquefaciens</i> N3-8 shows potential as a biocontrol agent against the pathogen <i>Burkholderia pseudomallei</i> and its effect on rice plantation.

ABSTRACT: Burkholderia pseudomallei is a saprophytic bacterium responsible for melioidosis in humans and animals. In this study, Bacillus amyloliquefaciens N3-8 was applied as a biocontrol agent on sterile soil spiked with 10⁷ colony-forming unit (CFU) per gram of B. pseudomallei p37 at two ratios: 1:10,000 and 1:100,000 CFU/g soil. Both treatments significantly reduced B. pseudomallei by 4-5 logs within 4 weeks. A subsequent experiment applied the 1:10,000 ratio to 10 kg of natural soil in a pot containing 10²-10³ CFU/g of B. pseudomallei alongside rice cultivation. Bacterial counts, rice yield, soil physicochemical factors, and microbial populations were monitored. B. pseudomallei was undetectable in biocontrol-treated soil

SUBMITTER: Potisap C 

PROVIDER: S-EPMC12131742 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature