Development of a culture-independent whole-genome sequencing of Nipah virus using the MinION Oxford Nanopore platform.

ABSTRACT: Nipah virus (NiV) is a deadly zoonotic pathogen in Southeast Asia causing severe respiratory and encephalitis symptoms with a high fatality rate. Whole-genome sequencing (WGS) is crucial for tracking transmission, conducting epidemiological analyses, and understanding NiV's adaptive evolution. WGS is essential for analyzing genomes, particularly in understanding pathogen nature, and pathogenesis and aiding in the development of therapeutics. However, sequencing this highly contagious virus directly from samples is challenging in low- and middle-income countries lacking BSL-4 facilities. This study developed and optimized a culture-independent, high-throughput multiplex PCR-based third-generation sequencing protocol for NiV using the Oxford Nanopore Technology platform and a proposed bioinf

SUBMITTER: Rahman MM 

PROVIDER: S-EPMC12131749 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature