Multiplex real-time reverse transcription recombinase-aided amplification assay for the detection of SARS-CoV-2, influenza A virus, and respiratory syncytial virus.

ABSTRACT: Influenza A virus (IAV), severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and respiratory syncytial virus (RSV) are significant pathogens that induce respiratory symptoms in humans, significantly impacting healthcare systems, economic activities, and human life on a global scale. The clinical manifestations associated with SARS-CoV-2, IAV, and RSV exhibit similarities. Cases of clinical coinfection are prevalent, highlighting the necessity for rapid differential diagnosis to avoid treatment delays. Traditional reverse transcription quantitative polymerase chain reaction (RT-qPCR) methods are often time-consuming and limited to central laboratories, necessitating the development of rapid and in situ screening techniques to accommodate broad and large-scale testing requir

SUBMITTER: Zhou Y 

PROVIDER: S-EPMC12131773 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature