Differences between human male and female neutrophils with respect to which RNAs are present in polysomes.

ABSTRACT:

Background

Human males and females show differences in the incidence of neutrophil-associated diseases such as systemic lupus erythematosus, rheumatoid arthritis, and multiple sclerosis, and differences in neutrophil physiological responses such as responses to infection, tissue damage, and chemotactic factors. However, little is known about the basis of sex-based differences in human neutrophils.

Methods

Starting with human neutrophils from healthy donors, we used RNA-seq to examine total RNA profiles, RNAs not associated with ribosomes and thus not being translated, RNAs in monosomes, and RNAs in polysomes and thus heavily translated.

Results

There were sex-based differences in the levels of RNAs across free RNA, monosome, and polysome fractions. Male neutrophils ha

SUBMITTER: Pilling D 

PROVIDER: S-EPMC12132174 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature